All cell species are exposed to the mechanical cues existing in the microenvironment, and their biochemical response is highly related to these mechanical stresses imposed on them. However, conventional rigid sensors still have large restriction on characterizing the biomolecules released from dynamic deformed cells or soft tissues due to the lack of conformable interface. To this respect, we have pioneered the development of stretchable electrochemical sensor, which equipped with excellent mechanical compliance and could synchronously deform with cells and tissues. To meet various requirements, we are designing different functionalized stretchable sensing interface to achieve the dynamic monitoring of transient biochemical molecules in the primary of cell mechanotransduction. Since multiple mechanical forces could simultaneously trigger complicated signaling cascades, we are also endeavoring to integrate stretchable sensing arrays into organ-on-chips to further study mechanotransduction systematically.